Autoradiography

Whole body autoradiography (WBA) utilises radiolabeled compounds to assess tissue distribution of new chemical entities in small laboratory animals. The method yields information on specific pharmacokinetic issues - tissue distribution, uptake of compound in organs and tissues and routes of elimination. Images reveal the tissue localisation of radiolabel with a resolution sufficient to allow analysis of organ or tissue sub-sites. Digital image processing and radiolabeling standards enable (semi-) quantitative analysis of individual whole-body sections.

This technology is a powerful tool for exploratory purposes and to meet regulatory requirements that can be used in a number of ways, including:

  • Validation of target organ for pharmacology or for solving toxicology issues
  • Site specific drug localisation and retention
  • Penetration of blood-brain barrier and brain distribution
  • Penetration in tumors
  • Drug-drug interactions or drug metabolism

We offer: custom design studies with Carbon-14- or tritium labelled compounds on small animals and image analysis of tissue distribution. Light microscope autoradiography (micro-autoradiography) is another of our services. Our equipment includes: 

  • Whole-Body Macrotome (PMW)
  • Phosphor imager
  • Carbon -14 film detector for higher resolution