OSD
Developers need to select an appropriate solvent system for dissolving a sample. They should also choose a suitable separation mode, such as reversed phase chromatography or Hydrophilic Interaction Chromatography (HILIC). A detection principle should also be chosen – for example, for UV or visible light, detection wavelength should be selected. UV detection is preferred if the analytes contain a UV chromophore due to the widespread availability of UV detectors in QC laboratories.
In an article with Pharm Tech, our Analytical Chemist Anders Mörtberg, discusses the key stages to method development.